Connexin 32 Antibody / GJB1

• Catalog Number: orb2640067
• Category: Antibodies
• Description: This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: R5.21C
• Tested applications: ELISA
• Reactivity: Mouse, Rat
• Isotype: Rat IgG2a, kappa
• Immunogen: Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA: 1-5ug/ml for coating (order BSA/sodium azide-free format)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Connexin 32 antibody is available for research use only.
• UniProt ID: P28230
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Connexin 32 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE Analysis of Purified, BSA-Free Connexin 32 Antibody (clone R5.21C). Confirmation of Integrity and Purity of the Antibody.