Connexin 32 Antibody / GJB1

• Catalog Number: orb386047
• Category: Antibodies
• Description: This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane a-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: M12.13
• Tested applications: FACS, IF, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG
• Immunogen: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Western blot: 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Connexin 32 antibody is available for research use only.
• UniProt ID: P08034
• Storage: Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Connexin 32 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.