Connexin 32 Antibody / GJB1

• Catalog Number: orb2640065
• Category: Antibodies
• Description: This Ab recognizes a protein of 27-32kDa, identified as Connexin 32, also called Gap junction protein beta 1. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane a-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GJB1/1753
• Tested applications: IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG
• Immunogen: A recombinant human protein was used as the immunogen for the Connexin 32 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Connexin 32 antibody is available for research use only.
• UniProt ID: P08034
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the Kappa Light Chain antibody for each application should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human liver with Connexin 32 antibody (clone GJB1/1753). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE human tonsil with Connexin 32 antibody (clone GJB1/1753). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.