COMT Antibody

• Catalog Number: orb308834
• Category: Antibodies
• Description: COMT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human COMT recombinant protein (Position: G52-P271). Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunocytochemistry , 0.5-1μg/mlImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By HeatImmunohistochemistry (Frozen Section), 0.5-1μg/mlWestern blot, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 30037 MW
• UniProt ID: P21964
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Catechol O-methyltransferase;2.1.1.6;COMT;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of COMT using anti-COMT antibody.Lane 1:human K562 cell;2:human SH-SY5Y cell;3:human HepG2 cell;4:human A431 cell;5:human MCF-7 cell;6:rat liver tissue.

IHC analysis of COMT using anti-COMT antibody. COMT was detected in paraffin-embedded section of Mouse Lung Tissue.

IHC analysis of COMT using anti-COMT antibody. COMT was detected in paraffin-embedded section of Human Placenta Tissue.

IHC analysis of COMT using anti-COMT antibody. COMT was detected in paraffin-embedded section of Rat Lung Tissue.

IHC analysis of COMT using anti-COMT antibody.COMT was detected in immunocytochemical section of A549 Cell.

IHC analysis of COMT using anti-COMT antibody.COMT was detected in frozen section of human placenta tissue.

IHC analysis of COMT using anti-COMT antibody.COMT was detected in frozen section of mouse lung tissue.

IHC analysis of COMT using anti-COMT antibody.COMT was detected in frozen section of rat lung tissue.