COMT antibody

• Catalog Number: orb19316
• Category: Antibodies
• Description: Goat polyclonal antibody to COMT
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 30.0; 24.5
• Target: COMT (internal)
• Entrez: 1312
• Protein Sequence: QDIIPQLKKKYDVD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti COMT antibody, anti catechol-O-methyltransfer
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IE: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2ug/ml.IHC: In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration, 2-4µg/ml.WB: Approx 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human Cerebellum using COMT antibody

Western blot analysis of Human Testis lysate using COMT antibody

Immunohistochemical staining of Human Testis using COMT antibody

Immunohistochemical staining of Human Small Intestine using COMT antibody