Collagen Type IV antibody

• Catalog Number: orb867276
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen Type IV
• Species/Host: Rabbit
• Tested applications: ELISA, IF, IHC-P, RIA, WB
• Immunogen: Purified collagen type IV from murine tumour tissue
• Concentration: app. 1 mg/ml
• Dilution range: (purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
• Conjugation: Unconjugated
• Hazard Information: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
• UniProt ID: P02463
• Storage: Storage: -20°C
• Buffer/Preservatives: Affinity purified antibody lyophilized from phosphate buffered solution; no rAlbumin and preservative added!
• Note: For research use only
• Application notes: Positive Control: Mouse skin or liver. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night
• Expiration Date: 12 months from date of receipt.