Collagen IV antibody

• Catalog Number: orb345353
• Category: Antibodies
• Description: Collagen IV antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IF, IHC, IP, WB
• Reactivity: Bovine, Human
• Isotype: IgG
• Immunogen: Collagen Type IV from human and bovine placenta
• Concentration: 1.17 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:50,000, IHC: 1:50 - 1:200, IF: User Optimized, IP: 1:100, WB: 1:1,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Conjugation: Unconjugated
• UniProt ID: P02462
• NCBI: NP_001290039.1
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Collagen Type IV antibody, Arresten an
• Note: For research use only
• Application notes: Anti-Collagen Type IV has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
• Expiration Date: 12 months from date of receipt.