Collagen IV antibody

• Catalog Number: orb19732
• Category: Antibodies
• Description: Goat polyclonal antibody to Collagen IV
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 70.8; 60.0
• Target: COL4A3BP (aa662-672)
• Entrez: 10087
• Protein Sequence: DHDSAPLNNRC
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-Arresten antibody, anti-COL4A1 antibody, anti
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in mouse heart lysates (calculated MW of 71.1kDa according to mouse NP_075909.1 and 60.0kDa according to human NP_112729.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse Heart lysate using Collagen IV antibody.