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Catalog Number | orb413033 |
---|---|
Category | Antibodies |
Description | CLPP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human CLPP recombinant protein (Position: A184-H247). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 26 kDa |
UniProt ID | Q16740 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | ATP-dependent Clp protease proteolytic subunit, mi Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-CLPP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of CLPP using anti-CLPP antibody.Lane 1:human PC-3 cell; 2:human HEK293 cell; 3:human MCF-7 cell; 4:human THP-1 cell; 5:rat pancreas tissue; 6:rat lung tissue; 7:mouse pancreas tissue.
IF analysis of CLPP using anti-CLPP antibody. CLPP was detected in immunocytochemical section of MCF-7 cells.
IHC analysis of CLPP using anti-CLPP antibody. CLPP was detected in paraffin-embedded section of human breast cancer tissue.
IHC analysis of CLPP using anti-CLPP antibody. CLPP was detected in paraffin-embedded section of human liver cancer tissue.
IHC analysis of CLPP using anti-CLPP antibody. CLPP was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of CLPP using anti-CLPP antibody. CLPP was detected in paraffin-embedded section of human ovarican cancer tissue.
IHC analysis of CLPP using anti-CLPP antibody. CLPP was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue.
ELISA, FC, ICC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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