CLPP antibody

• Catalog Number: orb18760
• Category: Antibodies
• Description: Goat polyclonal antibody to CLPP
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 30.2
• Target: CLPP
• Entrez: 8192
• Protein Sequence: EAAPAAEPVPAST
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CLPP antibody, anti ClpP caseinolytic proteas
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Liver shows patterned staining of hepatocyte cytoplasm. Recommended concentration, 2-4ug/ml.WB: Approx 27-30kDa band observed in human heart and skeletal muscle lysates (predicted MW of 32kDa according to NP_006003). Recommended for use at 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18760 staining (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18760 staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.