cleaved PARP Asp214 Antibody

• Catalog Number: orb1294351
• Category: Antibodies
• Description: Rabbit polyclonal antibody to cleaved PARP (Asp214)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide corresponding to residues surrounding Asp214 of human PARP
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin) 1:100-1:150
• Conjugation: Unconjugated
• Target: cleaved PARP Asp214
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-PARP celaved antibody - at 1/500 dilution. Lysates/proteins at 40 μg per lane. This blot was produced using a 5% and 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with orb1294351 overnight at 4°C.

Anti-PARP celaved antibody - at 1/500 dilution. Lysates/proteins at 40 μg per lane. This blot was produced using a 5% and 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with orb1294351 overnight at 4°C.

Anti-PARP celaved antibody - at 1/500 dilution. Lysates/proteins at 40 μg per lane. This blot was produced using a 5% and 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with orb1294351 overnight at 4°C.

Anti-PARP celaved antibody - at 1/500 dilution. Lysates/proteins at 40 μg per lane. This blot was produced using a 5% and 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with orb1294351 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294351 Anti-PARP cleaved antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PARP1 cleaved with orb1294351 at 1/100.

Immunohistochemical analysis of paraffin embedded Human oral carcinoma labeling PARP1 cleaved with orb1294351 at 1/150.