CLC4 Antibody

• Catalog Number: orb1263869
• Category: Antibodies
• Description: CLC4 Antibody
• Target: CLCN4
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Immunogen: This CLC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-689 amino acids from the C-terminal region of human CLC4.
• UniProt ID: P51793
• MW: 85 kDa
• Tested applications: IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H(+)/Cl(-) exchange transporter 4, Chloride channe
• Note: For research use only
• NCBI: P51793

Western blot of chicken brain tissue incubated with CLC4 Antibody.

Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody.

Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination.