CHRM2 Mouse Monoclonal Antibody

• Catalog Number: orb783415
• Category: Antibodies
• Description: CHRM2 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N8F12
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG1,Κ
• Immunogen: Recombinant human ChRM2.
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:200-500, IHC-P=1:25, IHC-F=1:25, ICC/IF=1:25, IF=1:25, Flow-Cyt=1:25
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: CHRM2
• UniProt ID: P08172
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 7TM receptor; Acetylcholine receptor, muscarinic,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: SH-SY5Y. Primary Antibody (green line): Mouse Anti-CHRM2 antibody (orb783415), dilution: 1:25, Isotype Control Antibody (blue line): Mouse IgG, Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1:400, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain section), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CHRM2) Monoclonal Antibody, Unconjugated (orb783415) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human heart section), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CHRM2) Monoclonal Antibody, Unconjugated (orb783415) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: SH-SY5Y cell lysates, Lane 2: Human brain tissue lysates, Lane 3: Mouse brain tissue lysates, Primary: Anti-CHRM2 (orb783415) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 55 kD.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CHRM2) monoclonal Antibody, Unconjugated (orb783415) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.