Chemiluminescent Western Blot Kit

• Catalog Number: orb342275
• Category: Assays and Kits
• Description: Chemiluminescent Western Blot Kit
• Conjugation: Unconjugated
• Reactivity: E. coli
• Purity: This Chemiluminescent Western Blot Kit allows for the detection of E.coli host cell proteins present in samples provided by the user. Included in the kit along with Rabbit Anti-Host Cell Protein antibody is a positive protein control, Goat Anti-Rabbit IgG HRP Antibody, and Blocking Buffer.
• Immunogen: Anti-Host Cell Protein Antibody (p/n 100-401-J07) was prepared blending antibodies that have been separately developed by repeated immunizations in rabbit with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli (BL21).
• Tested applications: WB
• Dilution range: WB: User Optimized by Kit Protocol
• Application notes: Use Biorbyt’ Chemiluminescent Western Blot Kit for detection of E.coli Host Cell Proteins. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-Host Cell Proteins Antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with Femtomax, a specialized formulation of luminol which is an extremely sensitive, non-radioactive substrate that emits light (chemiluminescence) and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Femtomax reagent (FEMTOMAX-020 or FEMTOMAX-110) is not included with this kit and must be purchased separately.
• Storage: See kit insert for complete instructions.
• Alternative names: Western Blotting Kit, Chemiluminescent Kit, Peroxi
• Note: For research use only

2D Western Blot of anti-E.coli HCP antibody. Load: 35 µg Total HCP. pH: 5-8. Primary antibody: Rabbit anti-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10000 for 30 min at RT. Block: orb348637 for 1 hour at RT.

Western Blot of Rabbit anti-HCP antibody. Lane 1: Molecular Weight. Lane 2: Total HCP. Load: 10 µg per lane. Primary antibody: Rabbit anti-HCP cocktail at 1:50 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10000 for 30 min at RT. Block: orb348637 for 1 hour at RT.