HRP Western Blot Rabbit IgG antibody (Peroxidase)

• Catalog Number: orb345944
• Category: Assays and Kits
• Description: HRP Western Blot Rabbit IgG antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: WB
• Concentration: 1.0 mg/ml
• Purity: HRP Western Blot Anti-Rabbit IgG Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: HRP
• Storage: See kit insert for complete instructions.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Anti-Rabbit IgG Peroxidase Conjugated Antibody for
• Note: For research use only
• Application notes: HRP Western Blot Anti-Rabbit IgG Antibody is specifically designed for immunoblotting used with Chemiluminescent Western Blot Kit (p/n KCA003) with Rabbit Primary Antibody.
• Expiration Date: 12 months from date of receipt.

(A) LoVo cells were either exposed to PTHα (30 µm) or transfected with non-specific siRNA (ns-siRNA) or p14ARF specific siRNA. Cells were treated with 2.5 µm oxaliplatin 8 h after siRNA or 1 h after PTHα treatment. 120 h upon oxaliplatin exposure, the expression of p14ARF, p21CIP1, and p53, as well as the phosphorylation of p53 at Ser15 was measured by immunodetection. HRP conjugated goat anti-mouse (p/n orb345943) and HRP conjugated goat anti-rabbit (p/n orb345944) were used.