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Catalog Number | orb613787 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to CD8 alpha |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | YTS 169.4 |
Tested applications | FC, IHC, IHC-Fr |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | Murine CD8 (Lyt-2). |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | CD8 alpha/Lyt-2 |
UniProt ID | P01731 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Leu2; CD8; CD8A; CD8a molecule; CD8a antigen; Leu2 Read more... |
Note | For research use only |
Application notes | The antibody has been shown to show depleting activity in vivo. Mice have two alleles for CD8, Lyt2.1, and Lyt2.2, which are restricted to certain mouse strains. Lyt2.1, for example, is expressed in the mouse strains CBA, AKR, C3H, and DBA, whereas Lyt2.2 is expressed in the mouse strains BALB/c and C57BL/6 (B/6). YTS 169.4 was originally raised against CBA mouse thymocytes. However, there are publications in which it has been used in C57BL/6 and BALB/c mice, wherein it was used specifically as a pan-reactive Lyt2 reagent (Tavaré et al., 2014; PMID: 24390540). |
Expiration Date | 12 months from date of receipt. |
IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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