CD71 Antibody

• Catalog Number: orb1264489
• Category: Antibodies
• Description: CD71 Antibody
• Target: TFRC
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This CD71 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human CD71.
• UniProt ID: P02786
• MW: 85 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Transferrin receptor protein 1, TR, TfR, TfR1, Trf
• Note: For research use only
• NCBI: P02786

Antibody staining CD71 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of CD71 Antibody in Hela cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with CD71 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of hela cells using CD71 Antibody (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.