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Catalog Number | orb1252796 |
---|---|
Category | Antibodies |
Description | CD68 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | C68/684 |
Tested applications | FC, IF, IHC |
Reactivity | Feline, Human, Monkey, Mouse, Rat |
Isotype | IgG1, kappa |
Immunogen | The subcellular fraction of human alveolar macrophages was used as the immunogen for this CD68 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CD68 |
UniProt ID | P34810 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Macrosialin, Gp110, CD68, CD68 Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of human tonsil (10X) stained with CD68 antibody (C68/684).
Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 antibody.
FACS, IF, IHC-P, WB | |
Feline, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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