CD63 Antibody

• Catalog Number: orb1671621
• Category: Antibodies
• Description: CD63 Antibody
• Clonality: Recombinant
• Clone Number: NK-1-C3
• Tested applications: FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD63
• UniProt ID: P08962
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Granulophysin, LAMP-3, Tetraspanin-30, Tspan-30, L
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed A431 cells with anti-CD63 antibody NK-1-C3. Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric r version of NK-1-C3 (orb1671621) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671621- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD63 antibody NK-1-C3. K562 cell lysate (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of NK-1-C3 (orb1671621) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.