CD63 Antibody

• Catalog Number: orb1252797
• Category: Antibodies
• Description: CD63 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MX-49.129.5
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1, kappa
• Immunogen: Full length human CD63 was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD63
• UniProt ID: P08962
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CD63 antigen, Granulophysin, Lysosomal-associated
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human melanoma stained with CD63 antibody (MX49.129.5).

IHC testing of mouse spleen stained with CD63 antibody (MX49.129.5).

FACS testing of human PBMC: Black = cells alone; Green = isotype control; Red = CD63 antibody PE conjugate.

FACS testing of mouse NIH3T3: Black = cells alone; Green = isotype control; Red = CD63 antibody PE conjugate.

Western blot testing of human spleen lysate with CD63 antibody at 2 ug/ml.

IHC testing of FFPE prostate carcinoma with CD63 antibody.

Immunofluorescence testing of HeLa cells with Alexa Fluor 488 conjugated CD63 antibody (green). F-actin filaments are labeled with Dylight 554 phalloidin (red); nuclei stained with DAPI (blue).