CD63 Antibody

• Catalog Number: orb1271031
• Category: Antibodies
• Description: CD63 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-190 amino acids from the C-terminal region of human CD63.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 26 kDa
• Target: CD63
• UniProt ID: P08962
• NCBI: P08962
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: CD63 antigen, Granulophysin, Lysosomal-associated
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Antibody staining CD63 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Overlay histogram showing HL-60 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H + L) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:2000 dilution + human plasma lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HL-60 whole cell lysates Lane 4: THP-1 whole cell lysates Lane 5: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: A2058 whole cell lysates Lane 2: MDA-MB-453 whole cell lysates Lysates/proteins at 20 ug per lane.

CD63 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.