CD6 Antibody

• Catalog Number: orb2642384
• Category: Antibodies
• Description: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular 'scavenger receptor cysteine-rich' (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: C6/2884R
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human recombinant protein was used as the immunogen for this recombinant CD6 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant CD6 antibody is available for research use only.
• UniProt ID: P30203
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant CD6 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with recombinant CD6 antibody (clone C6/2884R). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of human MOLT4 cells with CD6 antibody (clone C6/2884R, green) and Reddot nuclear stain (red).

Flow cytometry testing of human MOLT4 cells with CD6 antibody (clone C6/2884R); Red = isotype control, Blue = CD6 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant CD6 antibody (clone C6/2884R) as confirmation of integrity and purity.