CD49e antibody

• Catalog Number: orb1289703
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to CD49e
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein corresponding to human CD49e. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IF:IC: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ITGA5
• Entrez: 16402, 3678
• UniProt ID: P08648, P11688
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: FNRA; Integrin alpha-5; CD49 antigen-like family m
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD49e expression in NIH3T3 (A), HT1080 (B), rat heart (C) whole cell lysates. (Predicted band size: 114 kD; Observed band size: 135 kD)

Immunofluorescent analysis of CD49e staining in human placenta. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.