alpha 5 beta 1 Integrin antibody

• Catalog Number: orb610778
• Category: Antibodies
• Description: Recombinant monoclonal antibody to alpha 5 beta 1 Integrin
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: M200 (Volociximab)
• Tested applications: Blocking, ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: IgG4
• Immunogen: Heterodimeric α5β1Fc fusion protein
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: alpha 5 beta 1 Integrin
• UniProt ID: P08648
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Fibronectin receptor; CD49 antigen-like family mem
• Note: For research use only
• Application notes: The antibody binds specifically to α5β1 integrin, which is part of the superfamily of glycoprotein transmembrane receptors for ligands such as fibronectin, vitronectin, laminins and collagens. The α5β1 integrin receptor plays a key role in cellular processes such as inflammation, cell proliferation, angiogenesis and tumour metastasis. Comprising the variable domain of the original mouse antibody, a chimeric mouse:human IgG4 version of this antibody has been tested in vivo: In cynomolgus monkeys with laser-induced choroidal neovascularization (CNV), in rabbit models and in human volunteers. The antibody reached Phase II clinical trials with PDL Biopharma and Biogen-Idec.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of PBMC’s (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (blue line) using alpha 5 beta 1 Integrin antibody

Immunofluoresence staining of fixed HeLa cells using alpha 5 beta 1 Integrin antibody