CD45RA antibody

• Catalog Number: orb44329
• Category: Antibodies
• Description: Mouse Monoclonal to CD45RA.
• Clonality: Monoclonal
• Clone Number: MEM-56
• Tested applications: CyTOF, FC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Human thymocytes and T lymphocytes.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD45RA
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD45RA antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Mass cytometry (surface staining) of human peripheral blood with anti-CD45RA (MEM-56) 163Dy. Gated on singlets.

Western blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD45 control antibody; Lane 2: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under non-reducing conditions; Lane 3: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under reducing conditions.

Separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD45RA (MEM-56) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD45RA (MEM-56) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD45RA (MEM-56), 5 µg/ml.