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Catalog Number | orb443085 |
---|---|
Category | Antibodies |
Description | CD44 antigen CD44 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CD44 (728-742aa DETRNLQNVDMKIGV), different from the related mouse and rat sequences by one amino acid. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat Flow Cytometry, 1-3 μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 81538 MW |
UniProt ID | P16070 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | CD44 antigen;CDw44;Epican;Extracellular matrix rec Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HL-60 cells using anti-CD44 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of CD44 using anti-CD44 antibody.Lane 1:human HeLa cell;2:rat PC-12 cell.
IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.
IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in a paraffin-embedded section of human rectal cancer tissue.
IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in a paraffin-embedded section of rat lung tissue.
ELISA, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IHC-Fr, IHC-P, IP, LCI, WB | |
Human, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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