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| Catalog Number | orb1229852 |
|---|---|
| Category | Proteins |
| Description | CD40 Ligand Recombinant Protein |
| Target | CD40LG |
| Tag | Tag Free |
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Protein Sequence | Met113-Leu261 |
| UniProt ID | P29965 |
| MW | 16.2 kD |
| Application notes | This recombinant protein can be used for biological assays. For research use only. |
| Source | E. coli |
| Biological Activity | Recombinant CD40L is fully biologically active when compared to standards.ED50 is 5-10 ng/ml as determined by the dose-dependent stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells. |
| Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
| Alternative names | CD40 Ligand, CD40-L, T-Cell Antigen Gp39, TNF-Rela Read more... |
| Research Area | Cell Biology, Immunology & Inflammation, Signal Tr Read more... |
| Note | For research use only |
| NCBI | P29965 |
| Expiration Date | 6 months from date of receipt. |
Greater than 87% as determined by SDS-PAGE. | |
44.5 kDa | |
Mammalian cell |
> 95% as determined by SDS-PAGE and HPLC. | |
16.3 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
16.2 kDa | |
E.coli |
Unconjugated | |
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. | |
Predicted: 42.7 KDa. Observed: 50&90 KDa, reducing conditions |
Biotin | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 20.9 KDa. Observed: 23-28 KDa, reducing conditions |
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