CD4 antibody (APC)

• Catalog Number: orb179881
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD4 conjugated to APC
• Clonality: Monoclonal
• Clone Number: OX-35
• Tested applications: FC
• Reactivity: Rat
• Isotype: Mouse IgG2a kappa
• Immunogen: MLR generated rat Th cells
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-2 μg/ml.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: CD4
• Entrez: 24932
• UniProt ID: P05540
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 4 antibody (APC), anti CD4 (L3T4) antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of rat splenocytes stained using anti-rat CD4 (OX-35) APC antibody (concentration in sample 1.7 μg/ml).

Separation of rat CD4 positive cells (red-filled) from rat CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of rat splenocyte suspension stained using anti-rat CD4 (OX-35) APC antibody (concentration in sample 1.7 μg/ml).