CD154 Antibody (APC)

• Catalog Number: orb1806414
• Category: Antibodies
• Description: CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
• Clonality: Monoclonal
• Clone Number: 24-31
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: human CD154 fusion protein
• Conjugation: APC
• Target: CD154
• Entrez: 21947
• UniProt ID: P27548
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD154 (24-31) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent per million cells in 100 μl of cell suspension).

Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PHA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension).

Flow cytometry surface staining pattern of human stimulated (PHA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension).