CD34 Rabbit Polyclonal Antibody (Cy5)

• Catalog Number: orb914944
• Category: Antibodies
• Description: CD34 Rabbit Polyclonal Antibody (Cy5)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD34 (301-385/385aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IF=1:200-800
• Conjugation: Cy5
• Target: CD34
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD34 antigen; CD34 molecule; Cluster designation 3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-CD34 antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.