CD31 antibody

• Catalog Number: orb751176
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD31
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PECAM1/3534
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse / IgG2b, kappa
• Immunogen: Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)
• Concentration: 200 μg/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Conjugation: Unconjugated
• MW: ~100kDa (endothelium) and ~130kDa (platelets)
• Target: PECAM1
• Entrez: 5175
• UniProt ID: P16284
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-EndoCAM antibody, anti-PECA1 antibody, anti-P
• Note: For research use only
• Expiration Date: 12 months from date of receipt.