CD31 antibody

• Catalog Number: orb750532
• Category: Antibodies
• Description: CD31 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.
• Concentration: 90 mg/mL
• Dilution range: ELISA: 1:20,000, IHC: 4µg/mL, WB: 1:500
• Form/Appearance: Liquid (sterile filtered)
• Purity: CD31 PECAM1 antibody is directed against rat CD31 protein. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with CD31 from rat, human, and mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q3SWT0
• NCBI: NP_113779.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit anti-CD31 antibody, rabbit anti-PECAM-1 ant
• Note: For research use only
• Application notes: CD31 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to CD31 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
• Expiration Date: 12 months from date of receipt.