CD3 antibody

• Catalog Number: orb44592
• Category: Antibodies
• Description: Hamster monoclonal antibody to CD3
• Clonality: Monoclonal
• Clone Number: 145-2C11
• Tested applications: FA, FC, ICC, IHC-Fr, IP
• Reactivity: Mouse
• Isotype: Armenian Hamster IgG kappa
• Immunogen: Mouse BM10-37 cytotoxic T lymphocytes
• Concentration: 1 mg/ml
• Dilution range: Functional application: Induction of T cell activation, proliferation or apoptosis (depending on conditions), in vivo T cell depletion. Flow cytometry: Recommended dilution: 1-2 μg / ml (million cells). Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg protein in 1 ml of a cell lysate.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD3
• Entrez: 12501
• UniProt ID: P22646
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: anti CD 3 antibody, anti Cd247 antibody, anti CD24
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of murine CD3 positive splenocytes (red-filled) from CD3 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) purified antibody (low endotoxin, concentration in sample 4 μg/ml) DAR APC.

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) purified antibody (low endotoxin, concentration in sample 4 μg/ml) DAR APC.