CD3 Intracellular domain Antibody

• Catalog Number: orb1294242
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD3 (Intracellular
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human/mouse CD3
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:300-1:400 Immunohistochemistry (Paraffin) 1:100-1:300
• Conjugation: Unconjugated
• Target: CD3 Intracellular domain
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD3 antibody at 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 15% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294242 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Human Tonsil carcinoma tissue labeling CD3 with orb1294242 at 1/200.

Immunohistochemical analysis of paraffin embedded Human Hepatocellular cancer tissue labeling CD3 with orb1294242 at 1/100.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294242 anti-CD3 antibody at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Product: CD3 antibody; Catalog: orb1294242; Sample: Human peripheral blood; anti-CD3 antibody at 1:100.