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Catalog Number | orb44338 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD3 |
Clonality | Monoclonal |
Clone Number | MEM-57 |
Tested applications | FA, FC, IP, MC |
Reactivity | Human |
Isotype | Mouse IgG2a kappa |
Immunogen | Human thymocytes and T lymphocytes. |
Concentration | 1 mg/ml |
Dilution range | Immunoprecipitation: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7). Functional application: The antibody MEM-57 has strong mitogenic effect on peripheral T lymphocytes, it reacts strongly with gamma/delta T lymphocytes. Flow cytometry: Recommended dilution: 1-4 μg/ml; positive control: peripheral blood lymphocytes, JURKAT human leukemia T cell line. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | CD3 |
Entrez | 916 |
UniProt ID | P07766 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
Alternative names | anti CD 3 antibody, anti Cd247 antibody, anti CD24 Read more... |
Note | For research use only |
Application notes | Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications |
Expiration Date | 12 months from date of receipt. |
Mass cytometry analysis (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.
Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (MEM-57) purified antibody (low endotoxin, concentration in sample 0.33 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (MEM-57) purified antibody (low endotoxin, concentration in sample 0.33 μg/ml) GAM APC.
IF, IHC, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
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