CD3 Antibody

• Catalog Number: orb1294297
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD3
• Dilution range: Western Blot 1:500 – 1:1000 Immunofluorescence 1:300 – 1:400 Immunohistochemistry (Paraffin) 1:300 – 1:500 Flow Cytometry 1:100 – 1:200
• Conjugation: Unconjugated
• Target: CD3
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD3 antibody at 1/500 dilution, Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294297 overnight at 4°C.

Product: CD3 antibody; Catalog: orb1294297; Cell: Human peripheral blood; CD3 antibody: 1-100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD3 with orb1294297 at 1/400.

Cells were fixed with 4% PFA for 10 min, permeabilized with 0.1% NP-40 for 10 min then blocked with 5% BSA for 30 min. orb1294297 Anti-CD3 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.