CD3 antibody (Biotin)

• Catalog Number: orb44339
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD3 conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: MEM-57
• Tested applications: FC, IP
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: Human thymocytes and T lymphocytes.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2 - 5 μg/ml; positive control: peripheral blood lymphocytes, JURKAT human leukemia T cell line.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD3
• Entrez: 916
• UniProt ID: P07766
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 3 antibody (Biotin), anti Cd247 antibody (
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2 - 5 μg/ml; positive control: peripheral blood lymphocytes, JURKAT human leukemia T cell line.
• Expiration Date: 12 months from date of receipt.

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (MEM-57) biotin antibody (concentration in sample 4 µg/ml) streptavidin APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (MEM-57) biotin antibody (concentration in sample 4 µg/ml) streptavidin APC.