CD3 antibody (Biotin)

• Catalog Number: orb179872
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD3 conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: 145-2C11
• Tested applications: FC, ICC, IHC-Fr, IP
• Reactivity: Mouse
• Isotype: Armenian Hamster IgG kappa
• Immunogen: Mouse BM10-37 cytotoxic T lymphocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-2 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD3
• Entrez: 12501
• UniProt ID: P22646
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 3 antibody (Biotin), anti Cd247 antibody (
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of murine CD3 positive splenocytes (red-filled) from CD3 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) Biotin antibody (concentration in sample 8 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) Biotin antibody (concentration in sample 8 μg/ml, Streptavidin APC).