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Catalog Number | orb222827 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD3 conjugated to Biotin |
Clonality | Monoclonal |
Clone Number | UCHT1 |
Tested applications | FC, IHC-Fr, IP |
Reactivity | Human, Primate |
Isotype | Mouse IgG1 |
Immunogen | human thymocytes followed by Sezary T cells |
Concentration | 1 mg/ml |
Dilution range | Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-12 µg/ml. |
Purity | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
Conjugation | Biotin |
Target | CD3 |
Entrez | 916 |
UniProt ID | P07766 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti CD 3 antibody (Biotin), anti Cd247 antibody ( Read more... |
Note | For research use only |
Application notes | Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-12 µg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) biotin antibody (concentration in sample 0.3 μg/ml) streptavidin APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (UCHT1) biotin antibody (concentration in sample 0.3 μg/ml) streptavidin APC.
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