CD2AP Antibody

• Catalog Number: orb402259
• Category: Antibodies
• Description: CD2AP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human CD2AP recombinant protein (Position: K253-K337).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 85 kDa
• UniProt ID: Q9Y5K6
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: CD2-associated protein; Adapter protein CMS; Cas l
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-CD2AP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of CACO-2 cells using anti-CD2AP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of K562 cells using anti-CD2AP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD2AP using anti-CD2AP antibody.Lane 1:human K562 cell; 2:human HEK293 cell; 3:human A549 cell; 4:human HeLa cell; 5:human COLO320 cell; 6:rat liver tissue; 7:rat testis tissue; 8:mouse liver tissue; 9:mouse testis tissue.

IF analysis of CD2AP using anti-CD2AP antibody. CD2AP was detected in immunocytochemical section of K562 cells.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of rat kidney tissues.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of mouse kidney tissues.