CD2AP Antibody (monoclonal, 5F8)

• Catalog Number: orb507557
• Category: Antibodies
• Description: CD2AP Antibody (monoclonal, 5F8)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5F8
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: E. coli-derived human CD2AP recombinant protein (Position: K253-K337).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5 μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 80 kDa
• UniProt ID: Q9Y5K6
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: CD2-associated protein; Adapter protein CMS; Cas l
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ICC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in immunocytochemical section of A431 cell.Biotinylated goat anti-mouse IgG was used as secondary antibody.

Flow Cytometry analysis of K562 cells using anti-D2AP antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U20S cells using anti-CD2AP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD2AP using anti-CD2AP antibody.Lane 1:human K562 Cell;2:human A431 Cell;3:human HEK293 Cell;4:human U20S Cell;5:human HL-60 Cell;6:human MCF-7 Cell;7:human HeLa Cell;8:human PANC-1 Cell.

WB analysis of CD2AP using anti-CD2AP antibody.Lane 1:rat liver Tissue;2:rat testicular Tissue;3:mouse pancreas Tissue;4:mouse testicular Tissue;5:mouse RAW246.7 Cell.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human colon cancer.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human colon cancer.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human mammary cancer.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human mammary cancer.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human colon cancer.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human colon cancer.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of mouse kidney tissue.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of rat brain tissue.

IHC analysis of CD2AP using anti-CD2AP antibody.CD2AP was detected in paraffin-embedded section of rat kidney tissue.