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Catalog Number | orb1274248 |
---|---|
Category | Antibodies |
Description | CD1A Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | C1A/1506R |
Tested applications | FC, IF, IHC-P |
Reactivity | Human |
Isotype | IgG, kappa |
Immunogen | Full length human CD1a protein was used as the immunogen for this recombinant CD1a antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD1a antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CD1A |
UniProt ID | P06126 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | T-cell surface glycoprotein CD1a, T-cell surface a Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD1a antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human skin with recombinant CD1a antibody (clone C1A/1506R). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min.
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