CD1a antibody (PE)

• Catalog Number: orb43816
• Category: Antibodies
• Description: Mouse Monoclonal to CD1a.
• Clonality: Monoclonal
• Clone Number: HI149
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human thymocytes
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD1a
• Entrez: 909
• UniProt ID: P06126
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD1a antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using anti-human CD1a (HI149) PE antibody (20 µl reagent per milion cells in 100 µl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 µl reagent per milion cells in 100 µl of cell suspension).

Separation of human CD1a positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from CD11c negative CD1a negative events (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using CD1a (HI149) PE antibody (20 µl reagent per milion cells in 100 µl of cell suspension).

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using anti-human CD1a (HI149) PE antibody (20 µl reagent per milion cells in 100 µl of cell suspension).