CD1b Antibody (APC)

• Catalog Number: orb1806415
• Category: Antibodies
• Description: CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.
• Clonality: Monoclonal
• Clone Number: SN13
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: A cell membrane antigen preparation that was isolated from normal human thymocytes
• Conjugation: APC
• Target: CD1b
• Entrez: 910
• UniProt ID: P29016
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: R1
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using anti-human CD1b (SN13) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human CD11c positive CD1b positive cells (red-filled) from CD11c negative CD1b negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).