CD19 Antibody

• Catalog Number: orb1267924
• Category: Antibodies
• Description: CD19 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human CD19.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:500For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: CD19
• UniProt ID: P15391
• NCBI: P15391
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: B-lymphocyte antigen CD19, B-lymphocyte surface an
• Note: For research use only
• Application notes: For WB starting dilution is: 1:500For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from Ramos cell line, using CD19Antibody at 1:500.

Western blot analysis in Raji cell line lysates (35 ug/lane).

Western blot analysis of CD19 using rabbit polyclonal CD19 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2).

Immunofluorescence analysis of CD19 Antibody with paraffin-embedded human lymph tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Flow cytometric analysis of CEM cells using CD19 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.