CD19 Antibody

• Catalog Number: orb1248441
• Category: Antibodies
• Description: CD19 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-ENDSNLGQDQLS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kDa according to NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 75kDa
• Target: CD19
• UniProt ID: P15391
• NCBI: NP_001761.3, NP_001171569.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CD19, CD19 molecule, B4, CVID3, B-lymphocyte antig
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kDa according to NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248441 (2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.