CD171/L1CAM Rabbit Polyclonal Antibody

• Catalog Number: orb13325
• Category: Antibodies
• Description: CD171/L1CAM Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD171 (1101-1257/1257aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg /test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 138 kDa
• Target: L1CAM
• UniProt ID: P32004
• RRID: AB_10752378
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: L1CAM_HUMAN; Neural cell adhesion molecule L1; CAM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Antigen: orb1507790, 0.2 ug/100 ul, Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000, TMB staining, Read the data in MicroplateReader by 450nm.

Antigen: orb1507790, 0.2 ug/100 ul, Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000, TMB staining, Read the data in MicroplateReader by 450nm.

Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD171 antibody (orb13325), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA. Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.