CD16 antibody

• Catalog Number: orb222793
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD16
• Clonality: Monoclonal
• Clone Number: MEM-154
• Tested applications: FA, FC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human granulocytes
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody MEM-154 blocks binding of human IgG to FcγRIII. Flow cytometry: Recommended dilution: 5-10 μg/ml; positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Western blotting: Non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD16
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: FcgammaRIII
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of human neutrophil granulocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD16 (MEM-154) purified antibody (low endotoxin, concentration in sample 2 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD16 (MEM-154) purified antibody (low endotoxin, concentration in sample 2 μg/ml) GAM APC.