CD16/FCGR3A Antibody

• Catalog Number: orb402246
• Category: Antibodies
• Description: CD16/FCGR3A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human CD16 recombinant protein (Position: Q101-D166).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 45 kDa
• UniProt ID: P08637
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Low affinity immunoglobulin gamma Fc region recept
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of human PBMC cells using anti-CD16 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

IF analysis of CD16 using anti-CD16 antibody. CD16 was detected in an immunocytochemical section of K562 cells.

IHC analysis of CD16 using anti-CD16 antibody. CD16 was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of CD16 using anti-CD16 antibody. CD16 was detected in a paraffin-embedded section of human rectal cancer tissue.

IHC analysis of CD16 using anti-CD16 antibody. CD16 was detected in a paraffin-embedded section of human rectal cancer tissue.