CD16 antibody (PerCP)

• Catalog Number: orb154539
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to PerCP which recognizes CD16 that expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. It is a receptor for the Fc region of IgG. CD16 is one of only four eukaryotic receptors known to exist natively in both the transmembrane and glycosylphosphatidylinositol isoforms.
• Clonality: Monoclonal
• Clone Number: 3G8
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Human neutrophils
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PerCP
• Target: CD16
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-FcgammaRIII antibody, anti-IGFR3 antibody, an
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Staining pattern of Anti-human CD16 PerCP antibody (clone 3G8) in dot-plot fluorescence visualization (lymphocyte gate). Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 PerCP antibody (clone 3G8) was used in concentration 11 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD3 FITC antibody (clone UCHT1) in concentration 5 µg/ml, respectively.

Staining pattern of Anti-human CD16 PerCP antibody (clone 3G8) in dot-plot fluorescence visualization (mononuclear gate). Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 PerCP antibody (clone 3G8) was used in concentration 11 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD14 APC-Cy™7 antibody (clone MEM-15) in concentration 4 µg/ml, respectively.

Reactivity of Anti-human CD16 PerCP antibody (clone 3G8) on human peripheral leukocytes. Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Suspension of blood leukocytes (2 x 10^6 cells) was added to the mixture of CD16 PerCP antibody (clone 3G8, 11 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-human CD16 PerCP antibody (clone 3G8) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 PerCP antibody (clone 3G8) was used in concentration 11 µg/ml in stained blood sample (2 x 10^6 cells).